Apoptosis Detection Antibody Pack

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25080

Novus Biologicals, part of Bio-Techne
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NBP2-25080
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Key Product Details

Species

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot

Product Specifications

Specificity

The Apoptosis Detection Antbody Bundle contains monoclonal antibodies to three key apoptosis markers: caspase-3, caspase-8, and cleaved PARP. The caspase-3 and -8 antibodies both recognize pro and cleaved forms. The PARP antibody specifically recognizes the 89 kDa PARP fragment and does not recognized the full-length protein.

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Apoptosis Detection Antibody Pack

Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080]

Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080]

Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 (NB100-56708) in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080]

Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080]

Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Analysis of Caspase-8 in Jurkat cells using at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blot by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. this antibody was used in this test.
Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080]

Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080]

Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Analysis of cleaved PARP in staurosporin-treated Jurkat cells at various time points, using at 2 ug/ml. The band corresponding to cleaved PARP is only seen in the treated samples. anti-mouse Ig HRP conjugate was used in this test.

Formulation, Preparation, and Storage

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apoptosis Detection

Apoptosis, a form of programmed cell death, is among the most highly studied and analyzed processes in cell biology. The components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery and have central roles in the initiation and execution of apoptosis. Caspases are synthesized as inactive pro enzyme precursors (zymogens) of ~30-55 kDa with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspase activation is a hallmark of apoptotic signaling pathways. Caspases are activated early during apoptosis through proteolytic cleavage at specific asparagine residues by self-proteolysis and/or cleavage by other caspases. The asparagine resides are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases consisting of the heterotetramer p202-p102. The cleavage forms present are markers of caspase-dependent apoptosis. The reduction or loss of caspase proforms can also be a marker of caspase-dependent apoptosis. Caspase activation is followed by cleavage of a myriad of caspase substrates which contributes to the demise and eventual death of the cell. The most highly characterized caspase substrate is PARP. PARP [(ADP-ribose) polymerase] is a 113 kDa nuclear chromatin-associated enzyme that is involved in DNA repair and other cellular events. The cleavage of PARP into an 89 and 28 kDa fragment is considered indicative of functional caspase activation.

Alternate Names

apoptosis kinetics, apoptosis kit, apoptosis kit microscopy

Additional Apoptosis Detection Products

Product Documents for Apoptosis Detection Antibody Pack

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Certificate of Analysis

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Product Specific Notices for Apoptosis Detection Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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