Mouse IgG Horseradish Peroxidase-conjugated Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # HAF007
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Scientific Data Images for Mouse IgG Horseradish Peroxidase-conjugated Antibody
Detection of Mouse IgG Primary Antibody by Western Blot
Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Myocilin in human skeletal muscle
Detection of Myocilin in human skeletal muscle using 5 ug/mL of Mouse monoclonal anti-human Myocilin primary antibody (MAB3446) followed by 30 min incubation with Goat anti Mouse HRP conjugated secondary antibodies (Catalog # HAF007) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.Detection of Human IgG by Western Blot
Inverse correlation between the expression of MITF-M and that of miR-155 in melanoma cell lines incubated with IL-1 beta.(A) The expression of MITF-M, Tyrosinase and miR-155 was analyzed by quantitative RT-PCR in 8 melanoma cell lines incubated with IL-1 beta (10 ng/ml) for 4h or 24h. The results were normalized to beta-actin expression for MITF-M and Tyrosinase and to RNU44 for miR-155 (means ± SD for 3 independent experiments). (B) The expression of MITF-M and Tyrosinase proteins was analyzed by Western Blot in the same samples (1 of 3 independent experiments). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25853464), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse IgG Horseradish Peroxidase-conjugated Antibody
Immunohistochemistry
Western Blot
Use at a 1:1000 dilution.
Reviewed Applications
Read 15 reviews rated 4.7 using HAF007 in the following applications:
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Stability & Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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Product Specific Notices for Mouse IgG Horseradish Peroxidase-conjugated Antibody
For research use only