Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # J4H9

Format

BSA Free

Concentration

1.0 mg/ml

View all APP Antibodies »

Product Specifications

Immunogen

Recombinant human APP(18-289aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for APP Antibody (J4H9) - BSA Free

Western Blot: APP Antibody (J4H9)BSA Free [NBP1-04263]

Western Blot: APP Antibody (J4H9)BSA Free [NBP1-04263]

Western Blot: APP Antibody (J4H9) [NBP1-04263] - The lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse brain tissue lysate.
Immunocytochemistry/ Immunofluorescence: APP Antibody (J4H9) - BSA Free [NBP1-04263]

Immunocytochemistry/ Immunofluorescence: APP Antibody (J4H9) - BSA Free [NBP1-04263]

Immunocytochemistry/Immunofluorescence: APP Antibody (J4H9) [NBP1-04263] - Analysis of APP/Protease Nexin II in U87MG cells. The cell was stained using a dilution of (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: APP Antibody (J4H9) - BSA Free [NBP1-04263]

Flow Cytometry: APP Antibody (J4H9) - BSA Free [NBP1-04263]

Flow Cytometry: APP Antibody (J4H9) [NBP1-04263] - Flow analysis of APP/Protease Nexin II in 293T cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for APP Antibody (J4H9) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APP

Amyloid precursor protein (APP) is the precursor molecule whose proteolysis generates amyloid beta (Abeta), a 39- to 42- amino acid peptide and this amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's diseases patients. APP is an integral membrane protein that is phosphorylated in the cytoplasmic and extracellular domains. It has been reported that cell-surface APP plays a role in neurite extension of primary cultured hippocampal neurons. The large extracellular domain of APP is also reported to bind extracellular matrix molecules such as heparin, laminin, and collagen, which can mediate cell adhesion and neurite outgrowth. Abnormal regulation of the metabolism of APP may contribute to the deposition of plaques.

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

UniProt

NP_000475

Additional APP Products

Product Documents for APP Antibody (J4H9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for APP Antibody (J4H9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.