Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4C9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all APP Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human APP(NP_000475) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

85.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APP Antibody (OTI4C9) - Azide and BSA Free

Western Blot: APP Antibody (OTI4C9)Azide and BSA Free [NBP2-70197]

Western Blot: APP Antibody (OTI4C9)Azide and BSA Free [NBP2-70197]

Western Blot: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP.
Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197]

Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197]

Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Human lymphoma tissue.
Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197]

Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197]

Immunohistochemistry: APP Antibody (OTI4C9) - Azide and BSA Free [NBP2-70197] - Analysis of Adenocarcinoma of Human breast tissue.
View 9 more images

Applications for APP Antibody (OTI4C9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APP

Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (A 4) protein has been found to be the principal constituent of senile plaques of AD's patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa A 4 protein. Two variants of A 4 can be detected; A 1-40 "short-tailed" and A 1-42 "long-tailed" (3-4).

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

Additional APP Products

Product Documents for APP Antibody (OTI4C9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for APP Antibody (OTI4C9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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