APP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-308
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Rat
Predicted:
Canine (100%), Chicken (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P08592
Reactivity Notes
It is anticipated that the antibody will also work with canine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Localization
Cell Membrane, Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus
Specificity
Specific for endogenous levels of the ~115 kDa APP protein. Immunolabeling of the APP protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for APP Antibody
Western Blot: APP Antibody [NB300-308]
Western Blot: APP Antibody [NB300-308]Immunocytochemistry/ Immunofluorescence: APP Antibody [NB300-308]
Immunocytochemistry/Immunofluorescence: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) APP. Overlay images indicate the possible co-localization of VDR/APP on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.Western Blot: APP Antibody [NB300-308] -
Western Blot: APP Antibody [NB300-308] - Cell surface staining of VDR (green) on live neurons followed by fixation & immunofluorescence labeling of target proteins (100x): A) ADAM10; B) APP; C) BACE1; D) Nicastrin; E) Presenilin 1; F) Presenilin 2. Overlay images indicate the possible co-localization of VDR/ADAM10 (A) or VDR/APP (B) or VDR/Nicastrin (D) or limited co-localization of VDR/ Presenilin 1 (E) on the neuronal plasma membrane. Overlay images do not indicate co-localization for VDR/BACE1 (C) or VDR/Presenilin 2 (F) on the neuronal plasma membrane. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29176823), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for APP Antibody
Application
Recommended Usage
Western Blot
1:1000
Application Notes
Immunolabeling of the APP protein band is completely blocked by preadsorption of the with the peptide used as antigen. Expected band seen at ~115 kDa in Western blots. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 29176823).
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: APP
Long Name
Amyloid Precursor Protein
Alternate Names
Amyloid beta, beta Amyloid, Protease Nexin II
Gene Symbol
APP
UniProt
Additional APP Products
Product Documents for APP Antibody
Product Specific Notices for APP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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