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APPD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90124

Novus Biologicals, part of Bio-Techne
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NBP1-90124

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APPD Antibody

Western Blot: APPD Antibody [NBP1-90124]

Western Blot: APPD Antibody [NBP1-90124]

Western Blot: APPD Antibody [NBP1-90124] - Analysis in control (vector only transfected HEK293T lysate) and PLEKHF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: APPD Antibody [NBP1-90124]

Immunohistochemistry-Paraffin: APPD Antibody [NBP1-90124]

Immunohistochemistry-Paraffin: APPD Antibody [NBP1-90124] - Staining of human skeletal muscle shows distinct positivity in myocytes.

Applications for APPD Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APPD

APPD is composed of the pleckstrin homology domain and FYVE domain. It may induce apoptosis and is known to have interaction with TNNT1 and KIAA1279.

Alternate Names

Apoptosis-inducing protein, APPDapoptosis-inducing protein D, LAPF, Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains, MGC4090, PH and FYVE domain-containing protein 1, PH domain-containing family F member 1, PHAFIN1, phafin-1, pleckstrin homology domain containing, family F (with FYVE domain) member 1, pleckstrin homology domain-containing family F member 1, ZFYVE15phafin 1, Zinc finger FYVE domain-containing protein 15

Entrez Gene IDs

79156 (Human)

Gene Symbol

PLEKHF1

UniProt

Additional APPD Products

Product Documents for APPD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for APPD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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