APPL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57841

Novus Biologicals, part of Bio-Techne
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NB100-57841
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all APPL Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NRYSRLSKKRENDK corresponding to internal region according to NP_036228.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APPL Antibody

Western Blot: APPL Antibody [NB100-57841]

Western Blot: APPL Antibody [NB100-57841]

Western Blot: APPL Antibody [NB100-57841] - Staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL adenovirus for 48hrs using NB100-57841 at 0.5ug/ml. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.

Applications for APPL Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in (lane 1) rat aortic smooth skeletal muscle cells, (lane 2) intensity increased after infection with human APPL1 adenovirus for 48h (calculated MW of 79.7 kDa band according to NP_036228.1). The blot was co-labeled with Tubulin-specific antibodies as a loading control.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APPL

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.

Long Name

Adapter Protein Containing PH Domain PTB Domain and Leucine Zipper Motif 1

Alternate Names

APPL1, DIP13A

Entrez Gene IDs

26060 (Human); 72993 (Mouse)

Gene Symbol

APPL1

UniProt

Q9UKG1

Additional APPL Products

Product Documents for APPL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APPL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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