APPL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15434
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (99%), Porcine (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human APPL. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (89%), Zebrafish (86%), .
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for APPL Antibody
Western Blot: APPL Antibody [NBP2-15434]
Western Blot: APPL Antibody [NBP2-15434] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:1000.Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434]
Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - Mouse muscle. APPL1 antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434]
Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - AZ521 xenograft, using APPL1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for APPL Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: APPL
Long Name
Adapter Protein Containing PH Domain PTB Domain and Leucine Zipper Motif 1
Alternate Names
APPL1, DIP13A
Gene Symbol
APPL1
UniProt
Additional APPL Products
Product Documents for APPL Antibody
Product Specific Notices for APPL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.