Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Aprataxin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Aprataxin using the numbering given in SwissProt Entry Q7Z2E3 (GeneID 54840).

Specificity

NB100-534 is specific for human Aprataxin protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aprataxin Antibody

Western Blot: Aprataxin Antibody [NB100-534]

Western Blot: Aprataxin Antibody [NB100-534]

Western Blot: Aprataxin Antibody [NB100-534] - Detection of human Aprataxin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Aprataxin Antibody [NB100-534]

Western Blot: Aprataxin Antibody [NB100-534]

Western Blot: Aprataxin Antibody [NB100-534] - Whole cell lysate (50 ug) from normal human lymphoblasts (C3ABR cells; WT) or lymphoblasts from an AOA patient (738RM cells; AOA). Antibody used at 0.1 and 0.04 ug/ml.
Western Blot: Aprataxin Antibody [NB100-534]

Western Blot: Aprataxin Antibody [NB100-534]

Western Blot: Aprataxin Antibody [NB100-534] - Detection of Human Aprataxin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Aprataxin Antibody

Application
Recommended Usage

Immunoprecipitation

1 - 4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000. Immunoprecipitation: 1 to 4 ug/mg lysate. Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Aprataxin

This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined.

Alternate Names

AOA1EAOH, AOAMGC1072, aprataxin, ataxia 1, early onset with hypoalbuminemia, AXA1, EC 3.-, EOAHA, FHA-HIT, FLJ20157, Forkhead-associated domain histidine triad-like protein

Entrez Gene IDs

7332 (Human)

Gene Symbol

UBE2L3

UniProt

Q7Z2E3 (Human)

Additional Aprataxin Products

Product Documents for Aprataxin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aprataxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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