Aprataxin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98661

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Aprataxin Antibodies »

Product Summary for Aprataxin Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human Aprataxin fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Aprataxin Antibody

Western Blot: Aprataxin Antibody [NBP2-98661]

Western Blot: Aprataxin Antibody [NBP2-98661]

Western Blot: Aprataxin Antibody [NBP2-98661] - Anti-Aprataxin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa
Immunocytochemistry/ Immunofluorescence: Aprataxin Antibody [NBP2-98661]

Immunocytochemistry/ Immunofluorescence: Aprataxin Antibody [NBP2-98661]

Immunocytochemistry/Immunofluorescence: Aprataxin Antibody [NBP2-98661] - Immunofluorescence staining of Aprataxin in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Aprataxin polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661]

Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661]

Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661] - Immunochemical staining of human Aprataxin in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for Aprataxin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aprataxin

Aprataxin encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined.

Alternate Names

AOA1EAOH, AOAMGC1072, aprataxin, ataxia 1, early onset with hypoalbuminemia, AXA1, EC 3.-, EOAHA, FHA-HIT, FLJ20157, Forkhead-associated domain histidine triad-like protein

Gene Symbol

APTX

Additional Aprataxin Products

Product Documents for Aprataxin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aprataxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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