APRT Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92654

Novus Biologicals, part of Bio-Techne
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NBP2-92654-0.02ml
NBP2-92654-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all APRT Antibodies »

Product Summary for APRT Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1). MADSELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARHLKATHGGRIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLWASYSLEYGKAELEIQKDALEPGQRVVVVDDLLATGGTMNAACELLGRLQAEVLECVSLVELTSLKGREKLAPVPFFSLLQYE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APRT Antibody - BSA Free

Western Blot: APRT AntibodyBSA Free [NBP2-92654]

Western Blot: APRT AntibodyBSA Free [NBP2-92654]

Western Blot: APRT Antibody [NBP2-92654] - Analysis of extracts of various cell lines, using APRT at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Applications for APRT Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APRT

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

adenine phosphoribosyltransferase, AMP, AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, EC 2.4.2.7, MGC125856, MGC125857, MGC129961, transphosphoribosidase

Gene Symbol

APRT

Additional APRT Products

Product Documents for APRT Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for APRT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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