Skip to main content

Aquaporin-2 [p Ser269] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48314

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-48314

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Aquaporin-2 [p Ser269] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 conjugated to KLH. Accession # P34080

Modification

p Ser269

Specificity

This Antibody is specific for Aquaporin 2 (Ser 269).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aquaporin-2 [p Ser269] Antibody

Western Blot: Aquaporin-2 [p Ser269] Antibody [NBP1-48314]

Western Blot: Aquaporin-2 [p Ser269] Antibody [NBP1-48314]

Western Blot: Aquaporin-2 [p Ser269] Antibody [NBP1-48314] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser269.

Applications for Aquaporin-2 [p Ser269] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200 - 1:500

Immunohistochemistry

1:10-1:500

Immunoprecipitation

3 uL

Western Blot

1:1000-1:2000
Application Notes
WB analysis shows bands at 29/37 kDa
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aquaporin-2

Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.

Alternate Names

ADH water channel, AQP-2, AQP-CD, aquaporin 2 (collecting duct), aquaporin-2, Aquaporin-CD, Collecting duct water channel protein, MGC34501, Water channel protein for renal collecting duct, water-channel aquaporin 2, WCH-CD

Entrez Gene IDs

25386 (Rat)

Gene Symbol

AQP2

UniProt

Additional Aquaporin-2 Products

Product Documents for Aquaporin-2 [p Ser269] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aquaporin-2 [p Ser269] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...