I am from China and I would like to know more about your product number：NBL1-07638. We know that the theoretical MW of AQP3 protein is 31KD. But in your product specification, we can see that so many bands appear after overexpressing AQP3 with plasmid in 293 cell. Can you explain this phenomenon? Apart from theoretical strip, are all other nonspecific bands? Or is the AQP3 itself ?

Name: Aquaporin-3 Antibody
Brand: Novus Biologicals
SKU: NBP1-70376

Yes, the theoretical size of AQP3 is 31 kDa, which would be expected under reduced conditions with a sample. We have not characterized any of the other bands in the overexpressed lysate lane. Because this is a native lysate, any modifications that may take place could cause smearing and extra bands in the lysate sample. AQP3 is extensively modified because it is a glycoprotein, glycosylation often shows up as multiple bands with smearing when run under native conditions. This overexpression lysate is meant to only act as a positive control in WB experiments requiring a native control. Furthermore, the lane on the right side of the blot is showing detection of the lysate using an anti-Flag tag antibody, since the construct used for overexpression contains a Flag tag.

Aquaporin-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-70376

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

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Product Specifications

Immunogen

A synthetic peptide from the c-terminal region of rat Aquaporin-3 conjugated to blue carrier protein was used as the antigen.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This peptide is soluble in PBS and water.

Applications for Aquaporin-3 Antibody

Application

Recommended Usage

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Paraffin

1:300-1:2000

Western Blot

1:300-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin-3

AQP3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms

Alternate Names

AQP-3, Aquaglyceroporin-3, aquaporin 3, aquaporin 3 (GIL blood group), aquaporin 3 (Gill blood group), aquaporin-3, GIL, Gill blood group

Gene Symbol

AQP3

Additional Aquaporin-3 Products

Product Documents for Aquaporin-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Aquaporin-3 Antibody

This peptide is soluble in PBS and water.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Aquaporin-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-70376

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Aquaporin-3 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Applications for Aquaporin-3 Antibody

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Aquaporin-3

Alternate Names

Gene Symbol

Additional Aquaporin-3 Products

Product Documents for Aquaporin-3 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Aquaporin-3 Antibody

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