Aquaporin-3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97927
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
Reactivity Notes
Use in Rat reported in scientific literature (PMID:33634832).
Localization
Membrane
Specificity
Detects approx 31.5kDa. May detect larger glycosylated bands approx 35-50kDa.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aquaporin-3 Antibody
![Western Blot: Aquaporin-3 Antibody [NBP1-97927]](https://resources.bio-techne.com/images/products/Aquaporin-3-Antibody-Western-Blot-NBP1-97927-img0007.jpg "Western Blot: Aquaporin-3 Antibody [NBP1-97927]")
Western Blot: Aquaporin-3 Antibody [NBP1-97927]
Western Blot: Aquaporin-3 Antibody [NBP1-97927] - Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin-3 protein using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:2000.![Immunohistochemistry: Aquaporin-3 Antibody [NBP1-97927]](https://resources.bio-techne.com/images/products/Aquaporin-3 Antibody-Immunohistochemistry-NBP1-97927-img0008.jpg "Immunohistochemistry: Aquaporin-3 Antibody [NBP1-97927]")
Immunohistochemistry: Aquaporin-3 Antibody [NBP1-97927]
Immunohistochemistry: Aquaporin-3 Antibody [NBP1-97927] - Immunohistochemistry analysis using Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin-3 Polyclonal Antibody (NBP1-97927) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 m cryostat sections.Applications for Aquaporin-3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:400
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1:2000
Application Notes
0.5 ug/ml of Aquaporin 3 Antibody was sufficient for detection of aquaporin 3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aquaporin-3
Alternate Names
AQP-3, Aquaglyceroporin-3, aquaporin 3, aquaporin 3 (GIL blood group), aquaporin 3 (Gill blood group), aquaporin-3, GIL, Gill blood group
Entrez Gene IDs
65133 (Rat)
Gene Symbol
AQP3
UniProt
Additional Aquaporin-3 Products
Product Documents for Aquaporin-3 Antibody
Product Specific Notices for Aquaporin-3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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