Aquaporin-4 Antibody (5A4W4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35055
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 5A4W4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 256-323 of human Aquaporin-4 (AQP4) (NP_001641.1).
Sequence:
VEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV
Sequence:
VEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aquaporin-4 Antibody (5A4W4)
Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] -
Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] -
Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] -
Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Applications for Aquaporin-4 Antibody (5A4W4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aquaporin 4/AQP4
Long Name
Aquaporin 4
Alternate Names
AQP-4, AQP4, MIWC, WCH4
Gene Symbol
AQP4
Additional Aquaporin 4/AQP4 Products
Product Documents for Aquaporin-4 Antibody (5A4W4)
Product Specific Notices for Aquaporin-4 Antibody (5A4W4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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