Aquaporin-5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67247

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Porcine (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Aquaporin 5/AQP5 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of the human Aquaporin-5 protein (between amino acids 170-250). [UniProt P55064]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aquaporin-5 Antibody - BSA Free

Western Blot: Aquaporin-5 AntibodyBSA Free [NBP2-67247]

Western Blot: Aquaporin-5 AntibodyBSA Free [NBP2-67247]

Western Blot: Aquaporin-5 Antibody [NBP2-67247] - Total protein from human USO2, NTERA-2 and HeLa cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Aquaporin 5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Aquaporin-5 Antibody - BSA Free [NBP2-67247]

Immunocytochemistry/ Immunofluorescence: Aquaporin-5 Antibody - BSA Free [NBP2-67247]

Immunocytochemistry/Immunofluorescence: Aquaporin-5 Antibody [NBP2-67247] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-AQP5 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for Aquaporin-5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin 5/AQP5

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein aquaporin. Over the last decade, genes for several members of aquaporin family (AQP1-9 and AQPAP) have been cloned, expressed, and their distribution studied in many tissues. AQP5 (human, mouse, and rat 265aa) is found in several epithelial tissues (salivary and lachrymal glands, corneal epithelium in the eye and lung). It is implicated in generation of saliva, tears, and pulmonary secretions. AQP families of proteins are predicted to contain six transmembrane domains.

Long Name

Aquaporin 5

Alternate Names

AQP-5, AQP5, PPKB

Gene Symbol

AQP5

Additional Aquaporin 5/AQP5 Products

Product Documents for Aquaporin-5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aquaporin-5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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