Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A9/5

Concentration

0.2 mg/ml

View all ARF1 Antibodies »

Product Specifications

Immunogen

C-terminal human ARF1 peptide SNQLRNQ (Uniprot: P84077)

Localization

Cytoplasmic (Golgi Apparatus)

Marker

Golgi Apparatus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08340)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ARF1 Antibody (1A9/5)

Western Blot: ARF1 Antibody (1A9/5) [NBP3-07760]

Western Blot: ARF1 Antibody (1A9/5) [NBP3-07760]

Western Blot: ARF1 Antibody (1A9/5) [NBP3-07760] - Western Blot Analysis of human HeLa, HepG2 cell lysates and kidney tissue lysate using ARF1 Mouse Monoclonal Antibody (1A9/5).
Immunohistochemistry-Paraffin: ARF1 Antibody (1A9/5) [NBP3-07760]

Immunohistochemistry-Paraffin: ARF1 Antibody (1A9/5) [NBP3-07760]

Immunohistochemistry-Paraffin: ARF1 Antibody (1A9/5) [NBP3-07760] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (1A9/5).

Applications for ARF1 Antibody (1A9/5)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ARF1

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Long Name

ADP-ribosylation factor 1

Alternate Names

ADP-ribosylation factor 1

Gene Symbol

ARF1

UniProt

P84077 (Human)

Additional ARF1 Products

Product Documents for ARF1 Antibody (1A9/5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARF1 Antibody (1A9/5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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