ARF1,2,3,4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55421

Novus Biologicals, part of Bio-Techne
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NB100-55421
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Flow Cytometry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ARF1,2,3,4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EGLDWLSNQLRNQK, from the C Terminus of the protein sequence according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1.

Epitope

EGLDWLSNQLRNQK

Specificity

This antibody is expected to recognize all four reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ARF1,2,3,4 Antibody

Western Blot: ARF1,2,3,4 Antibody [NB100-55421]

Western Blot: ARF1,2,3,4 Antibody [NB100-55421]

Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of A431 (A) and (0.3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: ARF1,2,3,4 Antibody [NB100-55421]

Flow Cytometry: ARF1,2,3,4 Antibody [NB100-55421]

Flow Cytometry: ARF1,2,3,4 Antibody [NB100-55421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: ARF1,2,3,4 Antibody [NB100-55421]

Western Blot: ARF1,2,3,4 Antibody [NB100-55421]

Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of NIH3T3 (A), KNRK (B) and MDCK (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for ARF1,2,3,4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 18 kDa band observed in HeLa, A431, MCF7, NIH3T3, KNRK, and MDCK lysates (calculated MW of 20.7 kDa band according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARF1,2,3,4

DP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

ADP-ribosylation factor 1

Entrez Gene IDs

375 (Human); 11840 (Mouse); 64310 (Rat)

Gene Symbol

ARF1

UniProt

NP_001019397.1 (Human)

Additional ARF1,2,3,4 Products

Product Documents for ARF1,2,3,4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARF1,2,3,4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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