ARF5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15440
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for ARF5 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary.
Localization
Golgi apparatus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ARF5 Antibody
Immunohistochemistry-Paraffin: ARF5 Antibody [NBP2-15440] -
Immunohistochemistry-Paraffin: ARF5 Antibody [NBP2-15440] - ARF5 antibody detects ARF5 protein at cytoplasm in respiratory epithelium of mouse lung by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.
ARF5 antibody (NBP2-15440) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Western Blot: ARF5 Antibody [NBP2-15440]](https://resources.bio-techne.com/images/products/ARF5-Antibody-Western-Blot-NBP2-15440-img0003.jpg "Western Blot: ARF5 Antibody [NBP2-15440]")
Western Blot: ARF5 Antibody [NBP2-15440]
Western Blot: ARF5 Antibody [NBP2-15440] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.![Western Blot: ARF5 Antibody [NBP2-15440]](https://resources.bio-techne.com/images/products/ARF5-Antibody-Western-Blot-NBP2-15440-img0002.jpg "Western Blot: ARF5 Antibody [NBP2-15440]")
Western Blot: ARF5 Antibody [NBP2-15440]
Western Blot: ARF5 Antibody [NBP2-15440] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.Applications for ARF5 Antibody
Application
Recommended Usage
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ARF5
Additional ARF5 Products
Product Documents for ARF5 Antibody
Product Specific Notices for ARF5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.