Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ARFGEF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1800 and the C-terminus (residue 1849) of human brefeldin A-inhibited guanine nucleotide-exchange protein 1 using the numbering given in entry NP_006412.2 (GeneID 10565).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARFGEF1 Antibody

Western Blot: ARFGEF1 Antibody [NB100-55257]

Western Blot: ARFGEF1 Antibody [NB100-55257]

Western Blot: ARFGEF1 Antibody [NB100-55257] - Detection of Human and Mouse BIG1/ARFGEF1 on HeLa whole cell lysates using NB100-55257.
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257]

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257]

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for ARFGEF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARFGEF1

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.

Alternate Names

ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldinA-inhibited), ARFGEP1P200, BIG1p200, Brefeldin A-inhibited GEP 1, brefeldin A-inhibited guanine nucleotide-exchange protein 1, D730028O18Rik, DKFZP434L057, p200 ARF guanine nucleotide exchange factor, p200 ARF-GEP1

Entrez Gene IDs

10565 (Human); 211673 (Mouse)

Gene Symbol

ARFGEF1

UniProt

Q9Y6D6 (Human)

Additional ARFGEF1 Products

Product Documents for ARFGEF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARFGEF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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