ARFGEF1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55257
![Western Blot: ARFGEF1 Antibody [NB100-55257]](https://resources.bio-techne.com/images/products/ARFGEF1-Antibody-Western-Blot-NB100-55257-img0002.jpg "Western Blot: ARFGEF1 Antibody [NB100-55257]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1800 and the C-terminus (residue 1849) of human brefeldin A-inhibited guanine nucleotide-exchange protein 1 using the numbering given in entry NP_006412.2 (GeneID 10565).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ARFGEF1 Antibody - BSA Free
Western Blot: ARFGEF1 Antibody [NB100-55257]
Western Blot: ARFGEF1 Antibody [NB100-55257] - Detection of Human and Mouse BIG1/ARFGEF1 on HeLa whole cell lysates using NB100-55257.![Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257]](https://resources.bio-techne.com/images/products/ARFGEF1-Antibody-Immunohistochemistry-NB100-55257-img0004.jpg "Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257]")
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257]
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NB100-55257] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for ARFGEF1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:1000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ARFGEF1
Alternate Names
ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldinA-inhibited), ARFGEP1P200, BIG1p200, Brefeldin A-inhibited GEP 1, brefeldin A-inhibited guanine nucleotide-exchange protein 1, D730028O18Rik, DKFZP434L057, p200 ARF guanine nucleotide exchange factor, p200 ARF-GEP1
Gene Symbol
ARFGEF1
UniProt
Additional ARFGEF1 Products
Product Documents for ARFGEF1 Antibody - BSA Free
Product Specific Notices for ARFGEF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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