ARFIP1 Antibody (1F10)

Novus Biologicals, part of Bio-Techne | Catalog # H00027236-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARFIP1 Antibodies »

Product Specifications

Immunogen

ARFIP1 (NP_055262.1, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSL

Specificity

ARFIP1 - ADP-ribosylation factor interacting protein 1 (arfaptin 1) (1F10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ARFIP1 Antibody (1F10)

Western Blot: ARFIP1 Antibody (1F10) [H00027236-M05]

Western Blot: ARFIP1 Antibody (1F10) [H00027236-M05]

Western Blot: ARFIP1 Antibody (1F10) [H00027236-M05] - Analysis of ARFIP1 expression in transfected 293T cell line by ARFIP1 monoclonal antibody (M05), clone 1F10. Lane 1: ARFIP1 transfected lysatE (41.7 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: ARFIP1 Antibody (1F10) [H00027236-M05]

Immunohistochemistry-Paraffin: ARFIP1 Antibody (1F10) [H00027236-M05]

Immunohistochemistry-Paraffin: ARFIP1 Antibody (1F10) [H00027236-M05] - Analysis of monoclonal antibody to ARFIP1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
Sandwich ELISA: ARFIP1 Antibody (1F10) [H00027236-M05] - Detection limit for recombinant GST tagged ARFIP1 is 0.3 ng/ml as a capture antibody.

Applications for ARFIP1 Antibody (1F10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARFIP1

ADP-ribosylation factors, or ARFs, enhance the ADP ribosyltransferase activity of cholera toxin and are implicated in vesicle transport between endoplasmic reticulum and the Golgi complex. Arfaptin 1 is recruited from the cytosol to Golgi membranes by ARFs in a guanosine 5'-O-(3-thiotriphosphate)-dependent and brefeldin A-sensitive manner but is not a constituent of coatomer. Arfaptin 1 binds to nonmyristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1, another class I ARF. It binds with lower affinity to ARF5, a class II ARF, and with very little affinity to ARF6, a class III ARF. POR1 (also designated arfaptin 2) was first isolated as a Rac 1 binding protein necessary for Rac mediated Actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. POR1 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. The association of POR1 with ARF6 stimulates induction of Actin polymerization. POR1 appears to play a regulatory role through multiple signaling pathways in the reorganization of the cytoskeletal structure.

Alternate Names

ADP-ribosylation factor interacting protein 1, ADP-ribosylation factor-interacting protein 1, arfaptin 1, arfaptin-1, HSU52521, MGC117369

Entrez Gene IDs

27236 (Human)

Gene Symbol

ARFIP1

UniProt

NP_055262 (Human)

Additional ARFIP1 Products

Product Documents for ARFIP1 Antibody (1F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARFIP1 Antibody (1F10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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