ARFIP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87255
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MTDGILGKAATMEIPIHGNGEARQLPEDDGLEQDLQQVMVSGPNLNETSIVSGGYG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ARFIP2 Antibody
Western Blot: ARFIP2 Antibody [NBP1-87255]
Western Blot: ARFIP2 Antibody [NBP1-87255] - Analysis in control (vector only transfected HEK293T lysate) and ARFIP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: ARFIP2 Antibody [NBP1-87255]
Immunocytochemistry/Immunofluorescence: ARFIP2 Antibody [NBP1-87255] - Staining of human cell line A-431 shows positivity in nucleus, nucleoli and golgi apparatus. Antibody staining is shown in green.Immunohistochemistry-Paraffin: ARFIP2 Antibody [NBP1-87255]
Immunohistochemistry-Paraffin: ARFIP2 Antibody [NBP1-87255] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.Applications for ARFIP2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARFIP2
Alternate Names
ADP-ribosylation factor interacting protein 2, ADP-ribosylation factor-interacting protein 2, arfaptin 2, arfaptin-2, Partner of RAC1, POR1partner of RAC1 (arfaptin 2), Protein POR1
Entrez Gene IDs
23647 (Human)
Gene Symbol
ARFIP2
UniProt
Additional ARFIP2 Products
Product Documents for ARFIP2 Antibody
Product Specific Notices for ARFIP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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