ARG2 Antibody (1Y5U3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15697

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15697-100ul
NBP3-15697-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1Y5U3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Arginase 2/ARG2 Antibodies »

Product Summary for ARG2 Antibody (1Y5U3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 255-354 of human ARG2 (P78540). FDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARG2 Antibody (1Y5U3)

Western Blot: ARG2 Antibody (1Y5U3) [NBP3-15697]

Western Blot: ARG2 Antibody (1Y5U3) [NBP3-15697]

Western Blot: ARG2 Antibody (1Y5U3) [NBP3-15697] - Western blot analysis of extracts of 293T cells, using Arginase 2 (ARG2) antibody (NBP3-15697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: ARG2 Antibody (1Y5U3) [NBP3-15697]

Immunohistochemistry-Paraffin: ARG2 Antibody (1Y5U3) [NBP3-15697]

Immunohistochemistry-Paraffin: ARG2 Antibody (1Y5U3) [NBP3-15697] - Immunohistochemistry of paraffin-embedded human prostate cancer using Arginase 2 (ARG2) Rabbit mAb (NBP3-15697) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for ARG2 Antibody (1Y5U3)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Arginase 2/ARG2

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq]

Long Name

Kidney-Type Arginase

Alternate Names

ARG2, Arginase-2, Type II Arginase

Gene Symbol

ARG2

Additional Arginase 2/ARG2 Products

Product Documents for ARG2 Antibody (1Y5U3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARG2 Antibody (1Y5U3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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