Argonaute 3 Antibody (8E0O6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15939

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15939-100ul
NBP3-15939-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8E0O6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Argonaute 3 Antibodies »

Product Summary for Argonaute 3 Antibody (8E0O6)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 721-860 of human Argonaute 3 (Q9H9G7). RTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Argonaute 3 Antibody (8E0O6)

Western Blot: Argonaute 3 Antibody (8E0O6) [NBP3-15939]

Western Blot: Argonaute 3 Antibody (8E0O6) [NBP3-15939]

Western Blot: Argonaute 3 Antibody (8E0O6) [NBP3-15939] - Western blot analysis of extracts of various cell lines, using Argonaute 3 Rabbit mAb (NBP3-15939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: Argonaute 3 Antibody (8E0O6) [NBP3-15939]

Immunohistochemistry-Paraffin: Argonaute 3 Antibody (8E0O6) [NBP3-15939]

Immunohistochemistry-Paraffin: Argonaute 3 Antibody (8E0O6) [NBP3-15939] - Immunohistochemistry of paraffin-embedded human lung cancer using Argonaute 3 Rabbit mAb (NBP3-15939) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Argonaute 3 Antibody (8E0O6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Argonaute 3

Argonaute 3 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

AGO3argonaute 3, Argonaute3, eIF2C 3, eIF-2C 3, Eukaryotic translation initiation factor 2C 3, eukaryotic translation initiation factor 2C, 3, FLJ12765, hAgo3, MGC86946, protein argonaute-3

Gene Symbol

AGO3

Additional Argonaute 3 Products

Product Documents for Argonaute 3 Antibody (8E0O6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Argonaute 3 Antibody (8E0O6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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