ARHGAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85724

Novus Biologicals, part of Bio-Techne
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NBP1-85724
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARHGAP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SEALNQLKLASIDEKNWPSDEMPDFPKSDDSKSSSPELVTHLKWDDPYYDIARHQIVEVAGDDKYGRKIIVFSACRMPPSHQLDHSKLLGYLKHTLDQYVESDYTLLYLHHGLTSDNKPSLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGAP1 Antibody

Western Blot: ARHGAP1 Antibody [NBP1-85724]

Western Blot: ARHGAP1 Antibody [NBP1-85724]

Western Blot: ARHGAP1 Antibody [NBP1-85724] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ARHGAP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ARHGAP1 Antibody [NBP1-85724]

Immunocytochemistry/ Immunofluorescence: ARHGAP1 Antibody [NBP1-85724]

Immunocytochemistry/Immunofluorescence: ARHGAP1 Antibody [NBP1-85724] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Western Blot: ARHGAP1 Antibody [NBP1-85724]

Western Blot: ARHGAP1 Antibody [NBP1-85724]

Western Blot: ARHGAP1 Antibody [NBP1-85724] - Analysis in human cell line MCF-7.
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Applications for ARHGAP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1.0 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGAP1

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-boundstate. Cdc42 seems to be the preferred substrate

Long Name

Rho GTPase Activating Protein 1

Alternate Names

CDC42GAP, p50rhoGAP

Entrez Gene IDs

392 (Human)

Gene Symbol

ARHGAP1

UniProt

Q07960 (Human)

Additional ARHGAP1 Products

Product Documents for ARHGAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARHGAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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