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ARHGAP25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83053

Novus Biologicals, part of Bio-Techne
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NBP1-83053
NBP1-83053-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IQRVMTMMIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPEDSSKVPREK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARHGAP25 Antibody

Western Blot: ARHGAP25 Antibody [NBP1-83053]

Western Blot: ARHGAP25 Antibody [NBP1-83053]

Western Blot: ARHGAP25 Antibody [NBP1-83053] - Staining of human lymph node.
Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053]

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053]

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human cerebral cortex using Anti-ARHGAP25 antibody NBP1-83053.
Western Blot: ARHGAP25 Antibody [NBP1-83053]

Western Blot: ARHGAP25 Antibody [NBP1-83053]

Western Blot: ARHGAP25 Antibody [NBP1-83053] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Applications for ARHGAP25 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGAP25

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM]

Alternate Names

KAIA0053, KIAA0053, Rho GTPase activating protein 25, rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25

Entrez Gene IDs

9938 (Human)

Gene Symbol

ARHGAP25

UniProt

Additional ARHGAP25 Products

Product Documents for ARHGAP25 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARHGAP25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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