ARHGDIG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49854

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ARHGDIG Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EAVPEYRAPGRK corresponding to C-Terminus according to NP_001167.2.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARHGDIG Antibody

Western Blot: ARHGDIG Antibody [NBP1-49854]

Western Blot: ARHGDIG Antibody [NBP1-49854]

Western Blot: ARHGDIG Antibody [NBP1-49854] - Western blot analysis of ARHGDIG in HeLa lysate (35ug protein in RIPA buffer) using NBP1-49854 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ARHGDIG Antibody [NBP1-49854]

Immunohistochemistry-Paraffin: ARHGDIG Antibody [NBP1-49854]

Immunohistochemistry-Paraffin: ARHGDIG Antibody [NBP1-49854] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Applications for ARHGDIG Antibody

Application
Recommended Usage

Immunohistochemistry

0.5-2ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5-2 ug/ml
Application Notes
Western blot: Approx 25kDa band observed in lysates of cell line HeLa (calculated MW of 25.1kDa according to NP_001167.2). Primary incubation was 1 hour
IHC: Paraffin embedded Human Brain (Cortex).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARHGDIG

ARHGDIG inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms ofpost-translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammaliancells. Stimulates the release of the

Alternate Names

Rho GDI 3, Rho GDP dissociation inhibitor (GDI) gamma, rho GDP-dissociation inhibitor 3, RhoGDI gamma, Rho-GDI gamma, RHOGDI-3

Entrez Gene IDs

398 (Human); 14570 (Mouse); 360500 (Rat)

Gene Symbol

ARHGDIG

UniProt

Q99819 (Human)

Additional ARHGDIG Products

Product Documents for ARHGDIG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGDIG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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