ARHGEF5 Antibody (4D10-D11)

Novus Biologicals, part of Bio-Techne | Catalog # H00007984-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D10-D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARHGEF5 Antibodies »

Product Specifications

Immunogen

ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ARHGEF5 Antibody (4D10-D11)

Western Blot: ARHGEF5 Antibody (4D10-D11) [H00007984-M02]

Western Blot: ARHGEF5 Antibody (4D10-D11) [H00007984-M02]

Western Blot: ARHGEF5 Antibody (4D10-D11) [H00007984-M02] - Analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M02), clone 4D10-D11.Lane 1: ARHGEF5 transfected lysate (Predicted MW: 60.1 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: ARHGEF5 Antibody (4D10-D11) [H00007984-M02]

Immunoprecipitation: ARHGEF5 Antibody (4D10-D11) [H00007984-M02]

Immunoprecipitation: ARHGEF5 Antibody (4D10-D11) [H00007984-M02] - Analysis of ARHGEF5 transfected lysate using anti-ARHGEF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF5 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: ARHGEF5 Antibody (4D10-D11) [H00007984-M02] - Detection limit for recombinant GST tagged ARHGEF5 is 3 ng/ml as a capture antibody.

Applications for ARHGEF5 Antibody (4D10-D11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARHGEF5

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq]

Alternate Names

ephexin-3, GEF5, Guanine nucleotide regulatory protein TIM, Oncogene TIM, P60, p60 TIM, Rho guanine nucleotide exchange factor (GEF) 5, rho guanine nucleotide exchange factor 5, TIM1, TIMDKFZp686N1969, Transforming immortalized mammary oncogene

Gene Symbol

ARHGEF5

UniProt

AAH14555 (Human)

Additional ARHGEF5 Products

Product Documents for ARHGEF5 Antibody (4D10-D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGEF5 Antibody (4D10-D11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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