ARHGEF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2559

Novus Biologicals, part of Bio-Techne
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NB100-2559
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ARHGEF5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DKEIDQNSQQEE corresponding to internal region according to NP_005426.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ARHGEF5 Antibody

Western Blot: ARHGEF5 Antibody [NB100-2559]

Western Blot: ARHGEF5 Antibody [NB100-2559]

Western Blot: ARHGEF5 Antibody [NB100-2559] - (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NB100-2559]

Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NB100-2559]

Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NB100-2559] - (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ARHGEF5 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 170 kDa band observed in human HeLa lysates (calculated MW of 177 kDa band according to NP_005426.2).
IHC: Paraffin embedded Human Placenta.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARHGEF5

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Alternate Names

ephexin-3, GEF5, Guanine nucleotide regulatory protein TIM, Oncogene TIM, P60, p60 TIM, Rho guanine nucleotide exchange factor (GEF) 5, rho guanine nucleotide exchange factor 5, TIM1, TIMDKFZp686N1969, Transforming immortalized mammary oncogene

Entrez Gene IDs

7984 (Human)

Gene Symbol

ARHGEF5

UniProt

NP_005426.2 (Human)

Additional ARHGEF5 Products

Product Documents for ARHGEF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGEF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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