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ARHGEF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-23000

Novus Biologicals, part of Bio-Techne
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NBP1-23000

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human Rho guanine nucleotide exchange factor (GEF) 5 using the numbering given in entry NP_005426.2 (GeneID 7984).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGEF5 Antibody

Western Blot: ARHGEF5 Antibody [NBP1-23000]

Western Blot: ARHGEF5 Antibody [NBP1-23000]

Western Blot: ARHGEF5 Antibody [NBP1-23000] - Whole cell lysate from HeLa cells. ARHGEF5 was also immunoprecipitated by rabbit anti-ARHGEF5 antibody NBP1-23001.
Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NBP1-23000]

Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NBP1-23000]

Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NBP1-23000] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-APC7 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for ARHGEF5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
For IHC-P: Epitope retrieval with citrate buffer pH 6.0 is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARHGEF5

ARHGEF5, also known as TIM (transforming immortalized mammary), is an oncogene that belongs to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho GTPases. It is implicated to play a role in proliferative breast disease

Alternate Names

ephexin-3, GEF5, Guanine nucleotide regulatory protein TIM, Oncogene TIM, P60, p60 TIM, Rho guanine nucleotide exchange factor (GEF) 5, rho guanine nucleotide exchange factor 5, TIM1, TIMDKFZp686N1969, Transforming immortalized mammary oncogene

Entrez Gene IDs

7984 (Human)

Gene Symbol

ARHGEF5

UniProt

Additional ARHGEF5 Products

Product Documents for ARHGEF5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARHGEF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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