ARID1A Antibody (3611)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43566

Novus Biologicals, part of Bio-Techne
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NBP2-43566
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3611

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARID1A Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary.

Reactivity Notes

Rat (81%), Bovine (84%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

242 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARID1A Antibody (3611)

Western Blot: ARID1A Antibody (3611) [NBP2-43566]

Western Blot: ARID1A Antibody (3611) [NBP2-43566]

Western Blot: ARID1A Antibody (3611) [NBP2-43566] - Analysis of whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody [3611] at a dilution of 1:500.
Immunocytochemistry/ Immunofluorescence: ARID1A Antibody (3611) [NBP2-43566]

Immunocytochemistry/ Immunofluorescence: ARID1A Antibody (3611) [NBP2-43566]

Immunocytochemistry/Immunofluorescence: ARID1A Antibody (3611) [NBP2-43566] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.
Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566] - Mouse colon. ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for ARID1A Antibody (3611)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARID1A

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250a, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Gene Symbol

ARID1A

Additional ARID1A Products

Product Documents for ARID1A Antibody (3611)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARID1A Antibody (3611)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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