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ARID1A Antibody (HL2304) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25363

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25363

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2304

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for ARID1A Antibody (HL2304) - Azide and BSA Free

Immunogen

Recombinant fragment of human ARID1A.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID1A Antibody (HL2304) - Azide and BSA Free

ARID1A Antibody (HL2304) - Azide and BSA Free

Western Blot: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] -

Western Blot: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ARID1A Antibody (HL2304) - Azide and BSA Free

Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] -

Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] - ARID1A antibody [HL2304] detects ARID1A protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ARID1A stained by ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ARID1A Antibody (HL2304) - Azide and BSA Free

Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] -

Immunohistochemistry-Paraffin: ARID1A Antibody (HL2304) - Azide and BSA Free [NBP3-25363] - ARID1A antibody [HL2304] detects ARID1A protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ARID1A stained by ARID1A antibody [HL2304] (NBP3-25363) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for ARID1A Antibody (HL2304) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARID1A

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250a, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Gene Symbol

ARID1A

Additional ARID1A Products

Product Documents for ARID1A Antibody (HL2304) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARID1A Antibody (HL2304) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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