ARID1A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55334
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Zebrafish
Cited:
Mouse, Fish - Danio rerio (Zebrafish)
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (Negative), Western Blot
Cited:
Block/Neutralize, Chemotaxis, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human AT rich interactive domain 1A (BRG1-associated factor 250kD) using the numbering given in entry NP_006006.3 (GeneID 8289).
Reactivity Notes
Mouse, Zebrafish reactivity reported from verified customer reviews.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ARID1A Antibody
Western Blot: ARID1A Antibody [NB100-55334]
Western Blot: ARID1A Antibody [NB100-55334] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.1 ug/ml.
Chromatin Immunoprecipitation: ARID1A Antibody [NB100-55334] - In vivo Smarce1-binding sites were detected by chromatin immunoprecipitation using a SMARCE1 antibody. Cropped gel images representing typical results from ChIP assays in: the regulatory module located from the transcription initiation site +1 to -1415, which includes the basic promoter (gata5-Bp), the module located from -1469 to -1892 (gata5-A), the module located from -7823 to -8213 (gata5-B) and the module located from -16805 to -17147 (gata5-C). IgG, Smarce1, Smarca4, Arid1a and Pbrm indicate the PCR products from genomic DNA precipitated using the corresponding antibody. WT and Mut indicate the source of genomic DNA. The input is the PCR product from the total input genomic DNA. Full-length gels are presented in Supplementary Figure 3. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-33746-8), licensed under a CC-BY license.
Western Blot: ARID1A Antibody [NB100-55334]
Western Blot: ARID1A Antibody [NB100-55334] - Mouse embryonic fibroblasts nuclear extract at 1:1000 dilution. (Nuclear extract instead of whole cell lysate provides much better signal.) Image provided via verified customer review.Applications for ARID1A Antibody
Application
Recommended Usage
Western Blot
1:2000-1:10000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30337622). Immunoprecipitation Not recommended. ARID1A antibody validated for WB from verified customer reviews.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NB100-55334 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ARID1A
Alternate Names
ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250a, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1
Entrez Gene IDs
8289 (Human)
Gene Symbol
ARID1A
UniProt
Additional ARID1A Products
Product Documents for ARID1A Antibody
Product Specific Notices for ARID1A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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