ARID2 Antibody (7311)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43567

Novus Biologicals, part of Bio-Techne
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NBP2-43567
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7311

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARID2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

197 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARID2 Antibody (7311)

Knockout Validated: ARID2 Antibody (7311) [NBP2-43567]

Knockout Validated: ARID2 Antibody (7311) [NBP2-43567]

Knockout Validated: ARID2 Antibody (7311) [NBP2-43567] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody. (WT: wild type; KO: knockout)
Western Blot: ARID2 Antibody (7311) [NBP2-43567]

Western Blot: ARID2 Antibody (7311) [NBP2-43567]

Western Blot: ARID2 Antibody (7311) [NBP2-43567] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (7311) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ARID2 Antibody (7311) [NBP2-43567]

Immunocytochemistry/ Immunofluorescence: ARID2 Antibody (7311) [NBP2-43567]

Immunocytochemistry/Immunofluorescence: ARID2 Antibody (7311) [NBP2-43567] - detects ARID2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ARID2 stained by diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500. Scale bar= 10um.
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Applications for ARID2 Antibody (7311)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARID2

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID2 (AT-rich interactive domain-containing protein 2) is also known as BAF200 and was purified as a factor that associated with PBAF (hSWI/SNF-B). ARID2 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. In addition to the ARID domain, ARID2 also contains a C2H2-type zinc finger and has been implicated as a transcriptional co-activator for serum response factor for the regulation of cardiac genes.

Alternate Names

ARID domain-containing protein 2, AT rich interactive domain 2 (ARID, RFX-like), BAF200KIAA1557AT-rich interactive domain-containing protein 2, BRG1-associated factor 200, DKFZp686G052, DKFZp779P0222, FLJ30619, p200, Zinc finger protein with activation potential, zipzap, Zipzap/p200

Gene Symbol

ARID2

Additional ARID2 Products

Product Documents for ARID2 Antibody (7311)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARID2 Antibody (7311)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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