Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ARID3B Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 100 and 150 of human AT rich interactive domain 3B using the numbering given in entry EAW99328.1 (GeneID 10620).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID3B Antibody

Western Blot: ARID3B Antibody [NBP1-30456]

Western Blot: ARID3B Antibody [NBP1-30456]

Western Blot: ARID3B Antibody [NBP1-30456] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Affinity purified rabbit anti-ARID3B used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. ARID3B was also immunoprecipitated by rabbit anti-ARID3B antibodies NBP1-30455 and NBP1-30457, which recognize other epitopes.
Immunohistochemistry: ARID3B Antibody [NBP1-30456]

Immunohistochemistry: ARID3B Antibody [NBP1-30456]

Immunohistochemistry: ARID3B Antibody [NBP1-30456] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: ARID3B Antibody [NBP1-30456]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP1-30456]

Immunohistochemistry-Paraffin: ARID3B Antibody [NBP1-30456] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Applications for ARID3B Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
This antibody may be useful in Immunoflourescence/Immunocytochemistry as reported in the literature (PMID: 22860069)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARID3B

This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA-binding proteins. The encoded protein is homologous with two proteins that bind to the retinoblastoma gene product, and also with the mouse Bright and Drosophila dead ringer proteins. A pseudogene on chromosome 1p31 exists for this gene. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and possibly in chromatin structure modification.

Alternate Names

ARID domain-containing protein 3B, AT rich interactive domain 3B (BRIGHT- like), AT rich interactive domain 3B (BRIGHT-like), AT-rich interactive domain-containing protein 3B, BDP, bright and dead ringer protein, DRIL2

Gene Symbol

ARID3B

Additional ARID3B Products

Product Documents for ARID3B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARID3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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