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ARIH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1162

Novus Biologicals, part of Bio-Techne
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NB100-1162

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-HEGYEKDLWEYIED corresponding to C-Terminus according to NP_005735.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ARIH1 Antibody

Western Blot: ARIH1 Antibody [NB100-1162]

Western Blot: ARIH1 Antibody [NB100-1162]

Western Blot: ARIH1 Antibody [NB100-1162] - (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: ARIH1 Antibody [NB100-1162]

Immunohistochemistry-Paraffin: ARIH1 Antibody [NB100-1162]

Immunohistochemistry-Paraffin: ARIH1 Antibody [NB100-1162] - staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ARIH1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.2 - 1 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in U937 lysate (predicted MW of approx. 61 kDa band according to NP_005735).
IHC: Paraffin embedded Human Adrenal Gland.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARIH1

Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates

Alternate Names

ARI-1, ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1(Drosophila), ariadne, Drosophila, homolog of, ARIMOP-6, FLJ20329, FLJ93118, H7-AP2, HARI, Monocyte protein 6, MOP6, protein ariadne-1 homolog, UbcH7-binding protein, UBCH7BPariadne (Drosophila) homolog, ubiquitin-conjugating enzyme E2-binding protein1,HHARIDKFZp686O13120, UbcM4-interacting protein, Ubiquitin-conjugating enzyme E2-binding protein 1

Entrez Gene IDs

25820 (Human); 23806 (Mouse)

Gene Symbol

ARIH1

Additional ARIH1 Products

Product Documents for ARIH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARIH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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