ARIH2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1163
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EQRRRTLLKDFHDT corresponding to C-Terminus according to NP_006312.1.
Localization
Nuclear
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ARIH2 Antibody
![Western Blot: ARIH2 Antibody [NB100-1163]](https://resources.bio-techne.com/images/products/ARIH2-Antibody-Western-Blot-NB100-1163-img0001.jpg "Western Blot: ARIH2 Antibody [NB100-1163]")
Western Blot: ARIH2 Antibody [NB100-1163]
Western Blot: ARIH2 Antibody [NB100-1163] - Analysis of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer).Applications for ARIH2 Antibody
Application
Recommended Usage
Immunoprecipitation
1:10 - 1:500
Peptide ELISA
Detection limit 1:8000
Western Blot
1 - 3 ug/ml
Application Notes
IF: An anonymous customer has reported nuclear staining in DAPI.
IP: work well is noted. WB: Approx. 60 kDa band observed in nuclear lysates of Hela and K562 (calculated MW of 57.8 kDa band according to NP_006312.1). An anonymous customer has reported an approx. 60 kDa band in lysates of both human HeLa and mouse muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down (calculated MW of 57.8 kDa band according to NP_006312.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ARIH2
Alternate Names
ARI-2, ariadne (Drosophila) homolog 2, ariadne homolog 2 (Drosophila), E3 ubiquitin-protein ligase ARIH2, EC 6.3.2.-, FLJ10938, FLJ33921, Protein ariadne-2 homolog, Triad1 protein, TRIAD1ARI2all-trans retinoic acid inducible RING finger
Gene Symbol
ARIH2
UniProt
Additional ARIH2 Products
Product Documents for ARIH2 Antibody
Product Specific Notices for ARIH2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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