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ARIH2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49913

Novus Biologicals, part of Bio-Techne
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NBP1-49913

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for ARIH2 Antibody

Immunogen

The immunogen for this product maps to a region between residue 453 and 493 of human All-trans Retinoic Acid Inducible RING Finger using the numbering given in entry NP_006312.1 (GeneID 10425).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARIH2 Antibody

Western Blot: ARIH2 Antibody [NBP1-49913]

Western Blot: ARIH2 Antibody [NBP1-49913]

Western Blot: ARIH2 Antibody [NBP1-49913] - Detection of human and mouse Triad1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Triad1 antibody NBP1-49913 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: ARIH2 Antibody [NBP1-49913]

Immunoprecipitation: ARIH2 Antibody [NBP1-49913]

Immunoprecipitation: ARIH2 Antibody [NBP1-49913] - Detection of human Triad1 by western blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Triad1 antibody NBP1-49913 used for IP at 3 ug per reaction. Triad1 was also immunoprecipitated by another rabbit anti-Triad1 antibody. For blotting immunoprecipitated Triad1, NBP1-49913 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for ARIH2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARIH2

ARIH2 might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.

Alternate Names

ARI-2, ariadne (Drosophila) homolog 2, ariadne homolog 2 (Drosophila), E3 ubiquitin-protein ligase ARIH2, EC 6.3.2.-, FLJ10938, FLJ33921, Protein ariadne-2 homolog, Triad1 protein, TRIAD1ARI2all-trans retinoic acid inducible RING finger

Gene Symbol

ARIH2

Additional ARIH2 Products

Product Documents for ARIH2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARIH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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