ARK5/NUAK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36937

Novus Biologicals, part of Bio-Techne
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NBP1-36937
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ARK5/NUAK1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QNRPRPQYLKRYRNR corresponding to internal region according to NP_055655.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ARK5/NUAK1 Antibody

Immunocytochemistry/ Immunofluorescence: ARK5/NUAK1 Antibody [NBP1-36937]

Immunocytochemistry/ Immunofluorescence: ARK5/NUAK1 Antibody [NBP1-36937]

Immunocytochemistry/Immunofluorescence: ARK5/NUAK1 Antibody [NBP1-36937] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: ARK5/NUAK1 Antibody [NBP1-36937]

Flow Cytometry: ARK5/NUAK1 Antibody [NBP1-36937]

Flow Cytometry: ARK5/NUAK1 Antibody [NBP1-36937] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody

Applications for ARK5/NUAK1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:8000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARK5/NUAK1

NUAK1 is involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR

Long Name

AMPK-related Protein Kinase 5

Alternate Names

NUAK1

Entrez Gene IDs

9891 (Human); 299694 (Rat)

Gene Symbol

NUAK1

UniProt

NP_055655.1 (Human)

Additional ARK5/NUAK1 Products

Product Documents for ARK5/NUAK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARK5/NUAK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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