ARL2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1078
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-LDDISSRIFTAD corresponding to C-Terminus according to NP_001658.2.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Canine.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ARL2 Antibody
Western Blot: ARL2 Antibody [NB100-1078] -
Western Blot: ARL2 Antibody [NB100-1078] - (0.5ug/ml) staining of A431 (A) and (1ug/ml) HeLa nuclear (B) cell lysate (30ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: ARL2 Antibody [NB100-1078] -
Immunocytochemistry/Immunofluorescence: ARL2 Antibody [NB100-1078] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: ARL2 Antibody [NB100-1078] -
Flow Cytometry: ARL2 Antibody [NB100-1078] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for ARL2 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:64000
Western Blot
0.5-1 ug/ml
Application Notes
WB: Approx. 18kDa band observed in lysates of cell line A431 and approx. 20kDa in nuclear lysates of cell line HeLa (calculated MW of 20.9kDa according to NP_001658.2). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ARL2
Alternate Names
ADP-ribosylation factor-like 2, ADP-ribosylation factor-like protein 2, ARFL2
Entrez Gene IDs
402 (Human)
Gene Symbol
ARL2
UniProt
Additional ARL2 Products
Product Documents for ARL2 Antibody
Product Specific Notices for ARL2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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