Aromatase Antibody (1H1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26636
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1H1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Aromatase [UniProt P11511]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aromatase Antibody (1H1)
Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] -
Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - Image of Aromatase Antibody (1H1) diluted at 1:230 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot: Aromatase Antibody (1H1) [NBP3-26636] -
Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive Western Blot detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate.All lanes: Aromatase Antibody at 2.3ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 58, 25 KDa
Observed band size: 58 KDa
Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] -
Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of HepG2 cells with Aromatase Antibody (1H1) at 1:76, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).Applications for Aromatase Antibody (1H1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Aromatase
Aromatase is localized in the endoplasmic reticulum of the cell and its activity is regulated by tissue specific promoters that are in turn controlled by hormones, cytokines, and other factors. It can be found in many tissues reproductive tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, endometrium as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer.
Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function. The activity is increased by environmental factors such as age, obesity, insulin, gonadotropins, and alcohol but decreased by prolactin, anti-m llerian hormone, and smoking.
Long Name
Aromatase
Alternate Names
ARO1, CYAR, CYP19, CYP19A1, CYPXIX, EC 1.14.14.14, Estrogen synthase
Gene Symbol
CYP19A1
Additional Aromatase Products
Product Documents for Aromatase Antibody (1H1)
Product Specific Notices for Aromatase Antibody (1H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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