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Arylsulfatase A/ARSA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98210

Novus Biologicals, part of Bio-Techne
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NBP2-98210-50ul
NBP2-98210-100ul

Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse Arylsulfatase A/ARSA (Accession#: NP_033843.2; Met 1-Ser 506)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Arylsulfatase A/ARSA Antibody

Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210]

Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210]

Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Anti-Arylsulfatase A/ARSA rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Arylsulfatase A/ARSA Antibody [NBP2-98210]

Immunoprecipitation: Arylsulfatase A/ARSA Antibody [NBP2-98210]

Immunoprecipitation: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Mouse Arylsulfatase A/ARSA was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa

Applications for Arylsulfatase A/ARSA Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Arylsulfatase A/ARSA

ARSA is encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Multiple alternatively spliced transcript variants, one of which encodes a distinct protein, have been described for this gene. [provided by RefSeq]

Alternate Names

ARSA, Cerebroside 3-sulfatase

Gene Symbol

ARSA

Additional Arylsulfatase A/ARSA Products

Product Documents for Arylsulfatase A/ARSA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Arylsulfatase A/ARSA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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