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Arylsulfatase B/ARSB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86270

Novus Biologicals, part of Bio-Techne
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NBP1-86270
NBP1-86270-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Arylsulfatase B/ARSB Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YPGCGYWFPPPSQYNVSEIPSSDPPTKTLWLFDIDRDPEERHDLSREYPHIVTKLLSRLQFYHKHSVPVYF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Arylsulfatase B/ARSB Antibody

Immunocytochemistry/ Immunofluorescence: Arylsulfatase B/ARSB Antibody [NBP1-86270]

Immunocytochemistry/ Immunofluorescence: Arylsulfatase B/ARSB Antibody [NBP1-86270]

Immunocytochemistry/Immunofluorescence: Arylsulfatase B/ARSB Antibody [NBP1-86270] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-86270]

Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-86270]

Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-86270] - Staining in human kidney and salivary gland tissues using anti-ARSB antibody. Corresponding ARSB RNA-seq data are presented for the same tissues.
Immunohistochemistry: Arylsulfatase B/ARSB Antibody [NBP1-86270]

Immunohistochemistry: Arylsulfatase B/ARSB Antibody [NBP1-86270]

Immunohistochemistry: Arylsulfatase B/ARSB Antibody [NBP1-86270] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Applications for Arylsulfatase B/ARSB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Arylsulfatase B/ARSB

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ARSB, ASB, N-acetylgalactosamine-4-sulfatase

Gene Symbol

ARSB

Additional Arylsulfatase B/ARSB Products

Product Documents for Arylsulfatase B/ARSB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Arylsulfatase B/ARSB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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