ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49326

Novus Biologicals, part of Bio-Techne
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NBP2-49326
NBP2-49326-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASAH2/N-acylsphingosine Amidohydrolase-2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQNHQT

Reactivity Notes

Mouse (85%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]

Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]

Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]

Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]

Immunocytochemistry/Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.
Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]

Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]

Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Staining of human duodenum shows luminal membranous positivity in glandular cells.

Applications for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASAH2/N-acylsphingosine Amidohydrolase-2

The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in intestinal brush border membranes, but is also expressed in the kidney, brain and liver. ASAH2 is involved in the metabolism of dietary sphingolipids and the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

Long Name

Neutral Ceramidase

Alternate Names

Nacylsphingosine Amidohydrolase2

Gene Symbol

ASAH2

Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products

Product Documents for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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