ASB7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86157

Novus Biologicals, part of Bio-Techne
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NBP1-86157
NBP1-86157-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASB7 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HHHCRRNPELQEELQIQAAVAAGDVHTVRKMLEQGYSPNGRDANGWTLLHFSAARGKERCVRVFLEHGADPTVKDLIGGFTALHYAAMHGRARIARLMLESEYRSDIINAKSNDGWTPLHVAAHYGRDSFVRLLLEFKAEVDPLSDK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:33251222)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASB7 Antibody

Immunohistochemistry-Paraffin: ASB7 Antibody [NBP1-86157]

Immunohistochemistry-Paraffin: ASB7 Antibody [NBP1-86157]

Immunohistochemistry-Paraffin: ASB7 Antibody [NBP1-86157] - Staining in human testis and pancreas tissues using anti-ASB7 antibody. Corresponding ASB7 RNA-seq data are presented for the same tissues.
Western Blot: ASB7 Antibody [NBP1-86157]

Western Blot: ASB7 Antibody [NBP1-86157]

Western Blot: ASB7 Antibody [NBP1-86157] - Analysis in control (vector only transfected HEK293T lysate) and aSB7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ASB7 Antibody [NBP1-86157]

Immunocytochemistry/ Immunofluorescence: ASB7 Antibody [NBP1-86157]

Immunocytochemistry/Immunofluorescence: ASB7 Antibody [NBP1-86157] - Staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.
View 2 more images

Applications for ASB7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-P, Retrieval method: HIER pH6, ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASB7

ASB7 is a member of the ankyrin repeat and SOCS box family. Within the SOCS box of the ASB proteins are the BC and Cul5 boxes, via which ASB proteins including ASB7 interact with Cul5-Rbx2 as part of some E3 ubiquitin (Ub) ligase complexes. It is thought that the SOCS-containing ASB proteins function as the substrate recognition component of these complexes. E3 Ub ligases label proteins with Ub to target them for degradation by the proteasome.

Alternate Names

ankyrin repeat and SOCS box containing 7, ankyrin repeat and SOCS box protein 7, ankyrin repeat and SOCS box-containing 7, ASB-7, FLJ22551, FLJ25192

Gene Symbol

ASB7

Additional ASB7 Products

Product Documents for ASB7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASB7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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