ASB8 Antibody (OTI3A12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72400

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3A12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ASB8 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ASB8 (NP_077000) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

31.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASB8 Antibody (OTI3A12) - Azide and BSA Free

Western Blot: ASB8 Antibody (OTI3A12)Azide and BSA Free [NBP2-72400]

Western Blot: ASB8 Antibody (OTI3A12)Azide and BSA Free [NBP2-72400]

Western Blot: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASB8 .
Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400]

Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400]

Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)
Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400]

Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400]

Immunohistochemistry: ASB8 Antibody (OTI3A12) - Azide and BSA Free [NBP2-72400] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)

Applications for ASB8 Antibody (OTI3A12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ASB8

ASB8 (Ankyrin repeat and SOCS box protein 8) is a 288 amino acid protein that has its highest level of expression in skeletal muscle. It is also expressed in heart, brain, placenta, liver, kidney and pancreas. ASB8 contains a SOCS box domain suggesting that it may be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It also contains 4 ankyrin repeats which mediate protein-protein interactions and are among the most common structural motifs in known proteins.

Alternate Names

ankyrin repeat and SOCS box containing 8, ankyrin repeat and SOCS box protein 8, ankyrin repeat and SOCS box-containing 8, ASB-8, FLJ21255, MGC5540

Gene Symbol

ASB8

Additional ASB8 Products

Product Documents for ASB8 Antibody (OTI3A12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASB8 Antibody (OTI3A12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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