ASB9 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98662
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human ASB9 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ASB9 Antibody
Western Blot: ASB9 Antibody [NBP2-98662]
Western Blot: ASB9 Antibody [NBP2-98662] - Anti-ASB9 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).Immunocytochemistry/ Immunofluorescence: ASB9 Antibody [NBP2-98662]
Immunocytochemistry/Immunofluorescence: ASB9 Antibody [NBP2-98662] - Immunofluorescence staining of ASB9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ASB9 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Immunohistochemistry-Paraffin: ASB9 Antibody [NBP2-98662]
Immunohistochemistry-Paraffin: ASB9 Antibody [NBP2-98662] - Immunochemical staining of human ASB9 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.Applications for ASB9 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:500-1:2000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ASB9
Alternate Names
ankyrin repeat and SOCS box containing 9, ankyrin repeat and SOCS box protein 9, ankyrin repeat and SOCS box-containing 9, ankyrin repeat and suppressor of cytokine signaling box protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954
Gene Symbol
ASB9
Additional ASB9 Products
Product Specific Notices for ASB9 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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