ASCL3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68871
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HLPEDYLEKRLSK corresponding to internal region according to Uniprot Mouse NP_064435.1.
Epitope
C-HLPEDYLEKRLSK
Reactivity Notes
Expected from sequence similarity: Bovine
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ASCL3 Antibody
![Western Blot: ASCL3 Antibody [NBP1-68871]](https://resources.bio-techne.com/images/products/ASCL3-Antibody-Western-Blot-NBP1-68871-img0002.jpg "Western Blot: ASCL3 Antibody [NBP1-68871]")
Western Blot: ASCL3 Antibody [NBP1-68871]
Western Blot: ASCL3 Antibody [NBP1-68871] - (0.1ug/ml) Analysis of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871]](https://resources.bio-techne.com/images/products/ASCL3-Antibody-Immunohistochemistry-Paraffin-NBP1-68871-img0003.jpg "Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871]")
Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871]
Immunohistochemistry-Paraffin: ASCL3 Antibody [NBP1-68871] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for ASCL3 Antibody
Application
Recommended Usage
Immunohistochemistry
0.1-0.3ug/ml
Peptide ELISA
detection limit 1:8000
Western Blot
0.1 ug/ml
Application Notes
Western blot: Approx 23kDa band observed in Mouse Liver lysates (calculated MW of 20.2kDa according to NP_064435.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Brain (Cortex).
IHC: Paraffin embedded Human Brain (Cortex).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ASCL3
Alternate Names
achaete-scute complex homolog 3 (Drosophila), ASH-3, bHLH transcription factor Sgn-1 (Salivary Glands 1), bHLH transcriptional regulator Sgn-1, BHLHA42, bHLHa42achaete-scute homolog 3, Class A basic helix-loop-helix protein 42, hASH3, HASH3achaete-scute complex (Drosophila) homolog-like 3, Sgn1
Gene Symbol
ASCL3
UniProt
Additional ASCL3 Products
Product Documents for ASCL3 Antibody
Product Specific Notices for ASCL3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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