ASGPR1 Antibody (021)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89636
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 021
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ASGPR1 Antibody (021)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ASGPR1 (Uniprot#: P07306; Gln62-Leu291).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ASGPR1 Antibody (021)
Western Blot: ASGPR1 Antibody (021) [NBP2-89636]
Western Blot: ASGPR1 Antibody (021) [NBP2-89636] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDa Observed band size:43 kDaImmunoprecipitation: ASGPR1 Antibody (021) [NBP2-89636]
Immunoprecipitation: ASGPR1 Antibody (021) [NBP2-89636] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-ASGR1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-ASGR1 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDaApplications for ASGPR1 Antibody (021)
Application
Recommended Usage
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Application Notes
This antibody will detect Human ASGPR1 in ELISA pair set (Catalog: # NBP2-79552). In a sandwich ELISA, it can be used as a capture antibody when paired with (Catalog: # NBP2-89638).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ASGR1/ASGPR1
Long Name
Asialoglycoprotein Receptor 1
Alternate Names
ASGPR1, CLEC4H1, MHL1, RHL1
Gene Symbol
ASGR1
Additional ASGR1/ASGPR1 Products
Product Documents for ASGPR1 Antibody (021)
Product Specific Notices for ASGPR1 Antibody (021)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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