Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ASK1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1. The immunogen is located within amino acids 1260 - 1310 of ASK1. Amino Acid Squence: IEEKDQEIKHLKLKSQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASK1 Antibody - BSA Free

Western Blot: ASK1 AntibodyBSA Free [NBP1-76674]

Western Blot: ASK1 AntibodyBSA Free [NBP1-76674]

Western Blot: ASK1 Antibody [NBP1-76674] - SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
ASK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ASK1 Antibody - BSA Free [NBP1-76674] -

Immunocytochemistry/ Immunofluorescence: ASK1 Antibody - BSA Free [NBP1-76674] - Immunofluorescence of ASK1 in 3T3 cells with ASK1 antibody at 20 ug/mL.
ASK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ASK1 Antibody - BSA Free [NBP1-76674] -

Immunocytochemistry/ Immunofluorescence: ASK1 Antibody - BSA Free [NBP1-76674] - Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 2 u/mL.

Applications for ASK1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-20 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASK1

Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.

Long Name

Apoptosis Signal-regulated Kinase 1

Alternate Names

MAP3K5, MEKK5

Gene Symbol

MAP3K5

UniProt

BAA12684 (Human)

Additional ASK1 Products

Product Documents for ASK1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ASK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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